QUALITY OF LIFE AND MANAGEMENT OF
LIVING RESOURCES

ENVIRONMENTAL AGENTS SUSCEPTIBILITY ASSESSMENT
UTILISING EXISTING AND NOVEL BIOMARKERS AS RAPID
NON-INVASIVE TESTING METHODS

Contract n°QLK4-2002-02286

Project Progress summary

Reporting period I-XII 2003

Title of the project

ENVIRONMENTAL AGENTS SUSCEPTIBILITY ASSESSMENT UTILISING EXISTING AND NOVEL BIOMARKERS AS RAPID NON-INVASIVE TESTING METHODS

Acronym of the project: EASYRING

Type of contract: RTD

Total project cost: (in euro)

2351,056€

Contract number:

Duration: (in months)

EU contribution: (in euro)

QLK4-2002-02286

36Months

1890,209€

Commencement date:

01/01/2003

Period covered by the p. report:

1 January 2003 – 31 December 2003

PROJECT COORDINATOR

Name:

Alberta Mandich

Title:

Dr

Address: V.le Benedetto XV, 5 16132 Genoa, Italy

Telephone:

+390103538046

Telefax:

+390103538047

E-mail address:

mandich@unige.it

 

Key words: (5 maximum - Please include specific keywords that best describe the project.).

Endocrine disrupters, Biomarkers, Risk assessment, in vitro, dipstick

World wide web address: (the project’s www address )

www.easyring.org

 

List of participants
Provide all partners’ details including their legal status in the contract i.e.,contractor, assistant contractor (to which contractor?).

P1 and Coordinator Department of Environmental Experimental and Applied Biology (DI.BI.S.A.A.)
Team leader: Dr. Alberta MANDICH
Universityof Genoa, Viale Benedetto XV, 5 - 16132 Genoa - Italy
mandich@unige.it

P2 Department of Molecular Biology (UiB)
Team leader: Dr Bjørn-Einar GRØSVIK
University of Bergen, P.O. Box 7800, NO-5020 Bergen - Norway
bjorn-einar.grosvik@mbi.uib.no

P3 Biosense Laboratories AS (BIOSENSo)
Team leader: Prof. Anders GOKSØYR
Thormøhlensgate, 55 NO-5008 Bergen - Norway
Anders@biosense.no

P4 Department of Inland Fisheries (FWB.IGB)
Team leader: Prof. Werner KLOAS
Leibniz - Institute of Freshwater, Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin-Germany
werner.kloas@igb-berlin.de

P5 Water Research Institute National Council of Research (IRSA-CNR)
Team leader: Dr. Luigi VIGANO’
Via della Mornera, 25, 20047 Brugherio (Milan) - Italy
vigano@server-mi.irsa.rm.cnr.it
Subcontractor:
Dr. Gaetano Gentili
GRAIA Via Repubblica, 2, 21020 Varano Borghi (Varese) - Italy
graia@libero.it
Status: Private Institution

P6 Université de Mons-Hainaut (UMH)
Team leader: Prof. Henri ALEXANDRE
Faculté de Médicine et de Pharmacie, Service de Biologie et Embryologie, Avenue du Champ de Mars, 7
7000 Mons - Belgium
henri.alexandre@umh.ac.beSubcontractor:
Dr. Anne Van Cauwenberge
Institut Provincial d’Hygiène et de Bactériologie
Boulevard Sainctelette, 55
B-7000 Mons - Belgium
anne.vancauwenberge@hainaut.be
Status: Public (governmental) Institution

P7 Liverpool John Moores University (JMU)
Team leader: Dr. Mark CRONIN
School of Pharmacy and Chemistry, Byrom Street, Liverpool – L33AF, United Kingdom
M.T.Cronin@livjm.ac.uk

P8 Mario Negri Institute (NEGRI)
Team leader: Dr. Emilio BENFENATI
Laboratory of Environmental Chemistry and Toxicology, Via Eritrea, 62, 20157 Milan - Italy
benfenati@marionegri.it
Subcontractor:
Dr. Giuseppina Gini
Dipartimento di Elettronica e Informazione Politecnico di Milano, Via Ponzio, 34/5, 20133 Milan - Italy
gini@elet.polimi.it
Status: Public (governmental) Institution

Objectives:

The major objectives and achievements expected for EASYRING are:

  • Rapid, non-invasive test development for the identification of specific biomarkers for endocrine disrupting chemicals in the mucus of aquatic species.
  • Novel functional biomarker(s) for the identification of endocrine disrupting chemicals.
  • Production and validation of new enzyme linked immunosorbent assays (ELISAs) for the detection of new biomarkers in plasma, mucus and tissues of aquatic species.
  • Improvement of in vitro test for the screening of endocrine disrupting chemicals.
  • Development of analytical methods in GC-MS and LC-MS for selected endocrine disrupting chemicals in environmental water and biota.
  • Short/long term and low level exposure effects of selected chemicals and mixture in low vertebrates.
  • Short/long term and low level exposure effects of selected chemicals and mixture, through food and water chain, in small mammals.
  • QSARs development for the prediction of chemicals able to elicit endocrine disruption.
  • QAARs development to extrapolate the responses of the different experimental models.

Results and Milestones:

  • Identification of the fractions of environmental extracts showing the highest endocrine disrupting potentials when tested with the same battery of in vitro methods.
  • Development of new methods in GC-MS and LC-MS for selected endocrine disrupting chemicals in environmental water and biota (M17).
  • Development of assays on gamete maturation and activation (M20).
  • Protocol for identification ofshort/long term and low level exposure effects of selected chemicals and their mixtures in aquatic species (M3).
  • Protocol for identification short/long term and low level exposure effects of selected chemicals and their mixtures in small mammal (M4).
  • Pilot studies for the establishment of a specific protocol to speed up and simplify detection of candidate biomarkers in carps.
  • Initial protocol for immobilisation of antibodies in simple test system (M9).
  • Fish community characterisation in the Po river.
  • Establishment of retinol binding protein as new biomarker in Xenopus (M1)
  • Collation of data from the literature on endocrine disrupting activities and formulation of data-base (M25)
  • Brochure
  • Website

Benefits and Beneficiaries:

  • Improvement of the standard validated tests for risk assessment (beneficiaries: policy makers, scientist, national and regional environmental agencies, environmental quality monitoring agencies and laboratories)
  • Development of new non invasive test methods (beneficiaries: policy makers, scientist, national and regional environmental agencies, environmental quality monitoring agencies and laboratories)
  • Improvement of in vitro test systems with alternative screening and testing protocols (beneficiaries: scientist, environmental quality monitoring agencies and laboratories)
  • Improvement of chemical analytical method and their application (beneficiaries: scientist, environmental quality monitoring agencies and laboratories)
  • Improvement of knowledge on Cytotoxic and teratogenic effects of endocrine disrupters/modulators and quantification of exposures in mammals (cause-effects relationships investigation) (beneficiaries: policy makers, national and regional environmental and health agencies, scientist).

Future Actions (if applicable):

During the second year of the project, activities will be performed following the original timetable foreseen in the technical annex. Even though, due to the reproductive behaviour of carps (bounding to perform in vivo exposures at the end of summer to wait for the resting period), new biomarker(s) detection had been delayed, the Consortium set up strategies in order to solve the problem and close the gap. Moreover, during the first intermediate meeting, it was planned to perform a K1 carp caging in two sites located upstream and downstream the Lambro river confluence. This step was not foreseen in the original project, but it is considered a helpful tool to better understand data coming from the in lab exposures to chemicals selected on the base of in vitro testing and analytical analyses.

 

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